Cachectic Factor (CF) (Not for Export EU)

Size

£704.00

SKU:: C0104-49C
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide18-aa peptide of human CF
Format:: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Extra Details:: Cancer cachexia is a syndrome of progressive wasting which is believed to be mediated by tumor necrosis factor-alpha, interleukins 1 and 6, and interferon-gamma, and leukemia-inhibitory factor. However, the levels of these putative agents do not correlate with cachexia. Recently, a new circulating tumor factor, termed cachectic factor (CF), has been isolated and characterized from the urine of mice transplanted with adenocarcinoma MAC16 (1). Purified CF, a proteoglycan of relative mol. Wt of ~24kD, produces cachexia in vivo by inducing catabolism of skeletal muscle. CF was also found in urine of cachectic cancer patients. It is not found in normal urine, patient with weight loss due to trauma, and cancer patient with no weight loss (1). CF appears to be ~24kD on SDS-gels. It is a sulfated glycoprotein. The amino acid analyses was determined as: YSP EAA SAP GSG DPS HEA. An antibody raised to the CF also recognized a 69kD band with the same amino acid sequence as the 24kD material. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:500-2000 (ECL) ELISA: 1:10-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice