C0085-01D

CA50, Cancer Antigen (Colorectal Carcinoma Cell Line, COLO-205)

Size

£646.00

SKU:: C0085-01D
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CA50 protein.
Clone:: 10g164
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Cancer antigen 50 (CA50), a carcinoma associated carbohydrate antigen, was found to be elevated in the blood of patients with colorectal cancer and other malignancies. CA50 has been evaluated in various studies for use as a tumor marker for cancer diagnosis. Results showed that CS50 was marginally useful for the diagnosis and very useful for the follow up study of patients with pancreatic cancer. Applications: Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 10ug/ml ELISA: 0.2ng/ml can react with 1ug/ml coated ELISA plate wells Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile ddH2O. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.