C0050-15

CA125 Cancer Antigen, B Domain (Ovarian Cancer)

Size

£256.00

SKU:: C0050-15
Purity:: Purified by Ion Exchange chromatography from ascites. ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: CA125 antigen purified from human ovarian carcinoma (MW >1 million Daltons).
Clone:: 1.B.829 (X75)
Format:: Supplied as a liquid in 10mM Tris, 0.15M sodium chloride, pH 7.5, 0.05% sodium azide.
Extra Details:: According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.