C0050-01A-HRP

CA125 Cancer Antigen (Ovarian Cancer) (Glycerol Free) (HRP)

Size

£940.00

SKU:: C0050-01A-HRP
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Affinity purified human CA125 antigen.
Clone:: 2F1
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish peroxidase (HRP).
Extra Details:: According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of > 200kD and is detectable on the apical surface of normal endometrium and endocervix. Applications: Suitable for use in ELISA. Other applications not tested Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.