C0015-20
c-erbB2, p185 (HER2, neu Receptor)
Size
£927.00
- SKU:
- C0015-20
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to a region of the C-terminus of human c-erbB-2 protein. Cellular Localization: Cell membrane. Species Sequence Homology: rat, 100%
- Clone:
- 0.N.211
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C0015-20X.
- Extra Details:
- c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy. Applications: Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Denatured): 1:25 protein lysate using Protein G. Immunohistochemistry: 1:1000-1:2000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: SKBR-3 cells, MAD109 cells, breast carcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice