C0015-03B

c-erbB2, Recombinant, Human, aa679-1255, GST-Tag (HER 2, neu Receptor)

Size

£881.00

SKU:: C0015-03B
Molecular Weight:: 91.6
Purity:: As reported
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 20mM glutathione, 3mM DTT, 25% glycerol
Extra Details:: Human Her 2 (GenBank Accession No. X03363), (aa679-1255) with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: ≥40pmole/min/ug Unit Definition: One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na orthovanadate, 0.1mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate, and 10ug/ml recombinant Her2. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.