C0015-01M

c-erbB2 (Herstatin, V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, Neuro/glioblastoma derived oncogene homolog (avian) CD340, C-erbB-2, HER2, HER-2, HER-2/neu, MLN 19, NEU proto-oncogene, NGL, p185erbB2, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2)

Size

£800.00

SKU:: C0015-01M
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Thr23-Gly419 of human c-erbB2 expressed in Sf 21 cells.
Clone:: 9H75
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Herstatin is a secreted 68kD glycoprotein encoded by an alternate transcript of the HER-2/neu (ErbB-2) gene containing the N-terminal 304aa of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects c-erbB2 in paraffin-embedded human breast cancer tissue sections Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.