c-Cbl, phosphorylated (Tyr774)

Size

£639.00

SKU:: C0013-30
Purity:: Purified by protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to residues surrounding Tyr774 of human c-Cbl.
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Extra Details:: C-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in haematopoietic cells (1,2). c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules, such as protein tyrosine kinases, phosphatidylinositol 3 kinase, Crk and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain (TKB), a C3HC4 RING finger motif. TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. Its RING finger domain recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for c-Cbl proteins functioning as ubiquitin protein ligases and downregulation of RTKs (3). c-Cbl protein in human cancer tissues is frequently tyrosine phosphorylated in a tumor-specific manner (5). Tyr774 of c-Cbl is phosphorylated in Abl-transformed cells as well as T cells in response to pervanadate and to TcR/CD3-induced stimulation (4,6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice