C0013-02AX
c-Abl (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl) (BSA & Azide Free)
Size
£1,042.00
- SKU:
- C0013-02AX
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to c-Abl. Cellular Localization: Cytoplasmic and nuclear. Epitope: SH2 domain. MW of Antigen: 120kD.
- Clone:
- 2Q217 (8E9)
- Format:
- Supplied as a liquid in PBS, pH 7.4. Also supplied with BSA and sodium azide. See C0013-02A.
- Extra Details:
- Bcr-Abl hybrid gene product is a 210kD protein known to be assembled in K562 (human chronic myelogenous leukemia) cell line. c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both nucleus and cytoplasm of cells. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, c-abl proto-oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) as the result of the fusion of c-Abl sequences from chromosome 9 to Bcr gene on chromosome 22. The molecular consequence of translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: 2ug/mg protein lysate. Use Protein G. Western Blot: 1ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 12-24ug/ml for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells or placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice