C0010-17
C3a Receptor
Size
£524.00
- SKU:
- C0010-17
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- E. coli-expressed human C3a receptor fragment (amino acids 161-332, corresponding to its second extracellular loop)
- Format:
- Supplied as a lyophilized powder, 0.09% sodium azide. Reconsitute with 200ul sterile PBS.
- Extra Details:
- C3a is an anaphylatoxin generated through activation of the complement C3. C3a is a potent chemoattractant for phagocytes, and it stimulates chemotaxis and other leukocyte functions. C3a binds to eosinophils, basophils, neutrophils, and differentiated U937 cells, that all express receptors for C3a on their cell surface. The C3a receptor has been cloned and shown to be a G protein-coupled receptor with seven putative transmembrane domains1,2. This receptor is characterized for its large extracellular loop between the fourth and fifth transmembrane domains3. The loop can be readily detected using the rabbit anti-C3a receptor antibody. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: recognizes C3a receptor expressed in leukocytes and in transfected cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice