C2C12 Nuclear Extract, Differentiated (Muscle, Myoblast)

Size

£349.00

SKU:: C0010-13
Application:: WB
Purity:: Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Hepes, pH 7.9, 100mM KCl, 1mM MgCl2, 20% glycerol, 0.5mM PMSF, 0.5mM DTT.
Extra Details:: Permanent murine cell lines of fast and slow skeletal myocyte derivation. Positive Transcription Factors: Western Blot: Myf-4, Sp1, Sp3, TFIIB EMSA: E12/E47, Myf-3 (MyoD), Sp1 Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.