C0010-10B
C1q (11S Protein) (Biotin)
Size
£863.00
- SKU:
- C0010-10B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified mouse C1q.
- Clone:
- JL-1
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
- Extra Details:
- C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE. Antibody JL-1 was- administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used (Ref.1) Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 1:50 Western Blot: 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Spleen and kidney tissue of wild type mice Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice