C0010-02E-BIOTIN
Complement C1q (Complement Component 1, q Subcomponent) (Biotin)
Size
£1,075.00
- SKU:
- C0010-02E-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Globular head domain of Complement C1q, purified from human plasma.
- Clone:
- 3R9/2
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Human complement component 1, q subcomponent (C1q) is a 156kDa secreted protein. C1q associates with proenzymes C1r and C1s to form the calcium-dependent C1 complex, the first component of the serum complement system. C1q is composed of six A, six B-chains and six C-polypeptide chains. Each chain contains a collagen-like region located near the N-terminus and a C-terminal globular region. These regions bind the Fc region of IgM and IgG molecules, initiating the classical pathway of complement activation. C1q deficiency has been associated with lupus erythematosus and glomerulonephritis. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Positive Control: Kidney tissue from patients with streptococcal glomerulonephritis. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice