C0005-08

C-10 (MRP-1, Chemokine C-C motif ligand 6, CCL6)

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£668.00

SKU:: C0005-08
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse C-10, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul ddH2O.
Extra Details:: The chemokine C-10 is expressed by a range of cells including eosinophils, lung fibroblasts, some macrophages and myelopoietic mouse bone marrow cultures after GM-CSF activation. C-10 is chemotactic towards macrophages. Murine C-10 (MRP-1, Chemokine (C-C motif ligand 6, CCL6) is a member of the C-C chemokine family of proteins related to macrophage inflammatory protein-1 alpha (MIP-1 alpha). Murine C-10 is a 10.7kD protein containing 95 amino acid residues. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml, the detection limit for recombinant mC-10 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal working dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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