B5001-61H

BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743)

Size

£557.00

SKU:: B5001-61H
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to soluble human BTLA
Clone:: 11K3
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Extra Details:: Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1). Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis (3). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 Ficoll prepared human PBMC cells per tube were pelleted and pre-incubated for 5 minutes on ice with 20ul of human IgG at 300ug/ml. 2. Cells were washed and incubated for 45 minutes on ice with 80ul of B5001-61H at 5ug/ml 3. Cells were washed twice and incubated with 2º reagent reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using FACS. 4. Cells stained positive with a mean shift of 0.75 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. 5. Binding was blocked when reagent was pre-incubated with a 10-fold excess of recombinant CD-272-muIg (B5001-61M). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice