B3150-05C
BTF3 (Basic Transcription Factor 3, Nascent Polypeptide-associated complex, β/NACB)
Size
£837.00
- SKU:
- B3150-05C
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified, E. coli-derived recombinant human BTF3 rhBTF3; aa 2-162; Accession# P20290).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex β/NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ERα) stimulation of gene activation by binding to both ligand activated and non-ligand activated ERα. Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice