B2916
BSEP (Bile Salt Export Pump, SPGP, Sister of P-glycoprotein, ABCB11)
Size
£638.00
- SKU:
- B2916
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa705-718, YLVHEPPLAVVDHK of human BSEP (bile salt export pump).
- Clone:
- 6D172 (USal-hBSEP-McAb-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.7% BSA, 100mM sodium azide.
- Extra Details:
- BSEP is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominatly, if not exclusively, in the liver, where it is further localized to the canilicular microvilli and to subcanilicular vesicles fo the hepatocytes. Structurally, BSEP is a multifunctional polypeptide with two homologus halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Defects in BSEP are the cause of progressive familial intrahepatic cholestasis 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in BSEP are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Applications: Suitable for use in Immunocytochemistry and Western Blot. Not recommended for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:50-1:200 Immunocytochemistry: Cytospin preparations Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice