B2850
Bromodeoxyuridine (BrdU)
Size
£160.00
- SKU:
- B2850
- Molecular Weight:
- 307.1
- Storage Conditions:
- 4°C
- Supplier:
- United States Biological
- CAS Number:
- 59-14-3
- Extra Details:
- 5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations. Bromodeoxyuridine (BrdU) is also used to prepare dNTP-BrdU solution for UV cross-linking of proteins to nucleic acids and to prepare complete MEM-10-Brdu or MEM-20-Brdu for vaccinia virus stock cultures. Synonyms: 5-Bromo-2’-deoxyuridine; 5-BrdU; 2’-Deoxy-5-bromorouridine; (+)-5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; Bromodeoxyuridine; Broxuridine; NSC 38297 CAS No: 59-14-3 Molecular Formula: C9H11BrN2O5 Molecular Weight: 307.10 Purity (HPLC): ≥98% Appearance: White to off white powder Water (KF): ≤1% Method for Determining Identity: 1H NMR spectrometric analysis Melting Point: ~187-189°C (lit) DNase: None Detected RNase: None Detected Protease: None Detected Endotoxin: ≤0.5EU/mg Storage and Stability: Store at 4ºC. Stable for 12 months after receipt.
- Shipping Conditions:
- Ambient