B2850-14
Bromodeoxyuridine (BrdU)
Size
£800.00
- SKU:
- B2850-14
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bromodeoxyuridine-bovine serum albumin concentrate
- Clone:
- AH4H7-1/131-14871
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identifying proliferating cells in blood (1), tissues (2,3), tumors (4–7), as well as for determining plasma cell labeling indices (8). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 6ug/ml Flow Cytometry: 0.2ug/100ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice