B2850-10G-ML650
Bromodeoxyuridine (BrdU) (MaxLight 650)
Size
£856.00
- SKU:
- B2850-10G-ML650
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bromodeoxyuridine (BSA).
- Clone:
- Bu20a
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice