B2850-10G-HRP

Bromodeoxyuridine (BrdU) (HRP)

Size

£856.00

SKU:: B2850-10G-HRP
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bromodeoxyuridine (BSA).
Clone:: Bu20a
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques. Applications: Suitable for use in Immunohistochemistry (frozen, paraffin). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.