B2790-01A

BUB1 (Bub1, Bub-1, BublL, Budding uninhibited by benzimidazoles 1-like)

Size

£780.00

SKU:: B2790-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human BUB1.
Clone:: 14H5
Format:: Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Extra Details:: The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. Bub1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1ug/ml on tissue culture cells. Fix with 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation with blocking buffer is 10mM Tris, pH 7.5 with 150mM sodium chloride with 0.1% BSA. Immunoprecipitation: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.