B2739-70C

Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Size

£761.00

SKU:: B2739-70C
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mouse myeloma cells line NS0-derived, recombinant human Brevican isoform 1.
Clone:: 9L803
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml detects Brevican on rat cortical neural stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted B2739-70C overnight at 4°C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.