B2739-70B

Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Size

£812.00

SKU:: B2739-70B
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin <0.1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Asp23-Pro911 from human Brevican expressed in NS0 cells
Clone:: 8H196
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa52-139) and a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa649-911 that serves as a GPI-anchor motif. Over aa23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inhibition. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under non-reducing conditions only. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium. Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of biotinylated Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve >90% blocking in the assay. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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