BRCA1 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1) (Not for Export EU)

Size

£800.00

SKU:: B2709-03
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: GST-tagged fusion protein corresponding to aa1301-1863 of human BRCA1
Format:: Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Extra Details:: Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:10,000 dilution of this lot detected BRCA1 in HeLa nuclear extract. Immunohistochemistry: This antibody has been reported by an independent laboratory to detect BRCA1 in breast cancer tumor sections. Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate BRCA1. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.