B2704-25

Brain-Specific Receptor-like Protein A (BSRP-A)

Size

£761.00

SKU:: B2704-25
Purity:: Purified by Protein G affinity chromatography from cell culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Leu28-Ser837 from mouse BSRP-A, expressed in NSO cells Species Sequence Homology: human 96%, rat 99%.
Clone:: 9H71
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: BSRP-A (Brain-specific receptor-like protein A; also SEZ6L2) is a 170kD member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, and likely regulates PKC alpha activation. Mature mouse BSRP-A is an 890aa type I transmembrane protein. It contains an 824aa extracellular domain (ECD) (aa21-844) and a 43aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for BSRP-A. One shows a premature truncation after Arg403, a second shows a deletion of aa71-130, and a third likely represents the full-length form that contains a 13aa insertion after Val830. Over aa28-837, mouse BSRP-A is 96% and 99% aa identical to human and rat BSRP-A, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml with cell or tissue extracts. Conducted under reducing conditions Immunohistochemistry: 8-25ug/ml in perfusion fixed frozen sections of mouse brain (hippocampus) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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