B2704-12A

Brain-Specific Angiogenesis Inhibitor 3 (BAI3)

Size

£787.00

SKU:: B2704-12A
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ala25-Thr880 from human Brain-Specific Angiogenesis Inhibitor 3, expressed in NS0 cells. Uniprot/Accession Number: O60242
Clone:: 7H218
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Human BAI-3 (brain-specific angiogenesis inhibitor 3) is a 177kD, 7-transmembrane (TM) member of the secretin receptor family. It is synthesized by neurons of the CNS and likely is a negative regulator of angiogenesis. BAI-3 is 1498 amino acids (aa) in size. It contains three distinct regions; an N-terminal extracellular domain (ECD) (aa 25-883), a 7-TM segment, and a C-terminal cytoplasmic region. The ECD contains four antiangiogenic TSP type1 repeats (aa 296-508), and one GPS domain (aa 816-867) that is likely used to cleave the ECD from the membrane-bound receptor. There is one alternate splice form that shows a deletion of aa 643-665. Over aa 25-880, human BAI-3 is 98% aa identical to mouse BAI-3. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: Direct Western Blot: 1ug/ml using Recombinant Human BAI3 under non-reducing conditions only. Flow Cytometry: 0.25ug/10e6 cells using HEK293 Human Cell Line Transfected with Human BAI3 and eGFP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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