B2702-04W
Brain Natriuretic Peptide, Pro, NT (BNP)
Size
£863.00
- SKU:
- B2702-04W
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- E. coli-derived recombinant protein corresponding to His27-His134 from human proBNP.
- Clone:
- 8H160
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12kD BNP precursor gives rise to N-terminal proBNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa103-134) promotes vasodilation and fluid and sodium excretion. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~20ng/well for recombinant human BNP. Immunohistochemistry: 25ug/ml detects proBNP in paraffin-embedded normal human heart tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice