B2700-02A
Brain Derived Neurotrophic Factor (BDNF)
Size
£576.00
- SKU:
- B2700-02A
- Purity:
- Purified by immunoaffinity chromatography from serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Brain Derived Neurotrophic Factor expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Applications: Suitable for use in Neutralization, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Neutralization: 0.54-0.81ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of human Brain Derived Neurotrophic Factor (2ug/ml). ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human Brain Derived Neurotrophic Factor (B2700-02B) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human Brain Derived Neurotrophic Factor. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Brain Derived Neurotrophic Factor is 1.5-3ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry (FFPE): 2ug/ml staining U-2 OS and U-251 MG cells. B2700-02A was incubated overnight at 4ºC. followed by a flourescent labeled secondary antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice