B2673-76E

BRSK2 (Serine/Threonine-protein Kinase BRSK2, Brain-selective Kinase 2, Brain-specific Serine/Threonine-protein Kinase 2, BR Serine/Threonine-protein Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, SADA, STK29, HUSSY-12)

Size

£448.00

SKU:: B2673-76E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to CPEVIRGEKYDGRKAD, from BRSK2, at the internal region of the protein (NP_003948.2).
Format:: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Extra Details:: BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice