B2645
Bpl I
Size
£351.00
- SKU:
- B2645
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Extra Details:
- 5'-^ 8(N) G A G (N)5 C T C (N)13^-3' 3'-^13(N) C T C (N)5 G A G (N) 8^-5' BplI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase of BplI activity. The cleavage of DNA by BplI is never complete. Concentration: 5 units/ul Unit Definition: One unit is defined as the amount of Bpl I required to digest 1ug of lambda DNA-Xhol in 1 hour at 37°C in 50ul of assay buffer (a maximal cleavage level is achieved at which no change in the fragmentation pattern is observed with further increase of enzyme). Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Buffers Supplied: R1625 Restriction Enzyme Buffer A, 10X: 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA B2645-01: SAM 0.05mM S-adenosylmethionine Stability during Prolonged Incubation: A minimum of 0.3 units of Bpl I is required for complete digestion of 1ug of DNA in 16 hours at 37°C. Thermal Inactivation: Bpl I is inactivated by incubation at 65°C for 20min. Methylation Effect: DAM, DCM, EcoKl, EcoBl: never overlaps - no effect CpG: may overlap - no effect Number of Recognition Sites in DNA: Lambda: 1 PhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U: 0 Digestion of Agarose-embedded DNA: A minimum of 10 units of the Bpl I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BplI. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher thab L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Bpl I for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice