B2558-50

Bordetella pertussis

Size

£492.00

SKU:: B2558-50
Purity:: Purified by Protein A chromatography from ascites( >90%).
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bordetella pertussis.
Clone:: 5E222
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Extra Details:: Bordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens. Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28kD), S2 (MW 23kD), S3 (MW 22kD), S4 (MW 11.7kD) and S5 (MW 9.3kD), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:10-1:50 ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice