B2558-50-HRP

Bordetella pertussis (B. pertussis) (HRP)

Size

£832.00

SKU:: B2558-50-HRP
Purity:: Purified by Protein A chromatography ( >90%).
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bordetella pertussis
Clone:: 5E222
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Bordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens. Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28kD), S2 (MW 23kD), S3 (MW 22kD), S4 (MW 11.7kD) and S5 (MW 9.3kD), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface. Applications: Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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