B2557-01
Bora (Protein Aurora Borealis, HsBora, C13orf34)
Size
£806.00
- SKU:
- B2557-01
- Application:
- ICC
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to aa2-180 from human Bora expressed in E.coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Bora is a 61kD member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on aa210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates Bora dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559aa in length. It contains a Ser-rich region aa188-278 and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85kD. There is one potential alternative start site that lies 60aa upstream of the standard start site, and a second splice variant the shows a 17aa substitution for aa1-87. Over aa2-180, human Bora shares 84% aa identity with mouse Bora. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice