B2553-37A

Bone Morphogenetic Protein 10, Recombinant, Human (BMP10)

Size

£1,055.00

SKU:: B2553-37A
Molecular Weight:: 12.2
Purity:: ~97%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 30% Acetonitrile, 0.1% TFA, 1.25mg BSA. Reconstitute to 10ug/ml in sterile 4mM HCI, with ≥0.1% HSA or BSA.
Extra Details:: The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions. Source: Recombinant corresponding to Asn317-Arg424 from human BMP10 expressed in CHO cells (O95393). Activity: Measured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL. Reconstitution: It is recommended that sterile 4mM HCI containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/mL. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 4mM HCI, with ≥0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice