B2553-33H

Bone Morphogenetic Protein 7 (BMP7, OP-1, Osteogenic Protein-1) (Biotin)

Size

£479.00

SKU:: B2553-33H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human BMP-7, expressed in CHO cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to 0.1-1mg/ml.
Extra Details:: BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation. Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 0.25-1ug/ml (using 100ul/well). Used in conjunction with PB2553-33M as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBMP-7. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7/OP-1 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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