Bombesin Receptor 3 (BRS-3) (Not for Export EU)

Size

£704.00

SKU:: B2551-15
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 17aa peptide from the C-terminal cytoplasmic domain of human BRS3.
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: Bombesin (BN) is a tetradecapepide (Pyro-ENR LGN QWA VGH LM-amide) that was originally isolated from frog skin. Several BN-related peptides have been isolated from amphibians and classified into three subfamilies: Bombesin, ranatensin, and phyllolitorin based upon the last three C-terminal residues. Two BN-like peptides have been identified in mammals: NMB (neuromedin B) in the ranatensin family and GRP (gastrin-releasing peptide) in the BN-family. BN-family of G-protein coupled receptors include at least four receptor subtypes: The GRP-preferring receptors (GRP-R or bb2), the NMB-preferring receptors (NMB-R or bb1) and Bombesin receptor subtype 3 (BRS-3 or bb4). These receptors share approx. 50% amino acid homology and bind bombesin. However, BRS-3 has much lower affinity for BN than GRP-R and NMB-R. Mammalian BN-like peptides are widely distributed in the brain and gastrointestinal tract, where they modulate smooth-muscle contraction, exocrine and endocrine activities, metabolism and behavior. Most recently, BRS-3 deficient mice have been shown to develop mild obesity, associated with hypertension, and impairment of glucose metabolism, reduced metabolic rate, increased feeding efficiency and subsequent hyperphagia. Human BRS-3 gene (chromosome x) encodes a 399 aa protein with seven transmembrane domains. The NH2 and COOH-termini are predicted to be extracellular and cytoplasmic, respectively. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2500 ELISA: 1:5000-1:50,000. Using 50-100mg of control peptide (Catalog #B2551-40)/well Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice