Bnip3L (IN) Bnip3, Nix

Size

£655.00

SKU:: B2200
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to amino acids 77-92 (DAQHESGQSSSRGSSH) of human origin, which are identical to those of mouse Bnip3L.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Extra Details:: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-XL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml. A ~40kD top band represents the monomer of Bnip3L Optimal dilutions to be determined by the researcher. Related Products: Positive Control: K562 cell lysate; See Cat# K0001-25. Blocking Peptide: Available for competition studies; See Cat# B2200P. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice