B2185
Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)
Size
£615.00
- SKU:
- B2185
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-202 of mouse Bmi-1.
- Clone:
- 1.T.21 (F6)
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Bmi1 is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background, a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.2-2ug/ml. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with B2185. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Optimal dilutions to be determined by the researcher. Recommended Positive Control: U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice