Bmi-1 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)

Size

£607.00

SKU:: B2185-01E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide made to an internal region (within residues 1-100) of the human Bmi1 protein. Cellular Localization: Nuclear
Format:: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2, 0.05% sodium azide.
Extra Details:: The Bmi1 oncogene has been shown to induce telomerase activity and immortalize human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi1 is also important in maintaining stem cells' ability to self renew. It has also been termed an oncogene with aberrant expression in various carcinomas. It has been shown to be a negative regulator of p19Arf, Ink4a (p14Arf) and tp53 known tumor repressors. Ablation of Bmi1 is required for short term survival of cancer cells. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: A band is seen at ~37kD. Some less intense non-specific bands are also present. Optimal dilutions to be determined by the researcher. Positive Control: Human and mouse skeletal muscle Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice