Bmi-1 (B Lymphoma Mo-MLV Insertion Region, BUP, MGC12685, Murine Leukemia Viral (Bmi-1) Oncogene Homolog, Oncogene BMI-1, Polycomb Group RING Finger Protein 4, PCGF4, Polycomb Complex Protein BMI-1, RING Finger Protein 51, RNF51) (PE)

Size

£902.00

SKU:: B2185-01A-PE
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa peptide sequence near the centre of human BMI-1.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Extra Details:: BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.