Bmi-1 (B Lymphoma Mo-MLV Insertion Region, BUP, MGC12685, Murine Leukemia Viral (Bmi-1) Oncogene Homolog, Oncogene BMI-1, Polycomb Group RING Finger Protein 4, PCGF4, Polycomb Complex Protein BMI-1, RING Finger Protein 51, RNF51) (Biotin)

Size

£902.00

SKU:: B2185-01A-BIOTIN
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa peptide sequence near the centre of human BMI-1.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.