B2106-70C

Blimp-1 (BLIMP 1, BLIMP1, B Lymphocyte Induced Maturation Protein 1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1)

Size

£889.00

SKU:: B2106-70C
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Lys667-Cys789 from human Blimp-1, expressed in E. coli.
Clone:: 11D96
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Human Blimp-1 is a 91kD zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa64-170) and four C-terminal C2H2-type zinc-finger motifs (aa539-645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80kD, 691aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61-603 aa in length. Over aa 667-789, human Blimp-1 is 89% aa identical to mouse Blimp-1. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects Blimp-1 in U266 human myeloma cells. Flow Cytometry: 0.25ug/10e6 cells using U266 human myeloma cell line Immunocytochemistry: 8-25ug/ml detects Blimp-1 in immersion fixed U266 human myeloma cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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