B1750-34
Biotin (HRP)
Size
£335.00
- SKU:
- B1750-34
- Application:
- WB
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Biotin
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Applications: This product has been optimized to detect biotinylated protein markers (P9106-75) by Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:3000 Note: To detect other biotinylated proteins by Western Blot: 1:100 in PBS, 5% BSA, 0.05% Tween 20 with overnight incubation. Optimal dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Companion Products: P9106-75: Prestained Protein Marker, Broad Range P9106-25: Protein MW Marker, Detection (Biotin)
- Shipping Conditions:
- Blue Ice