B1750-30
Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (HRP)
Size
£1,209.00
- SKU:
- B1750-30
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with 1ml sterile ddH2O. Labeled with horseradish peroxidase (HRP)
- Extra Details:
- Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:25,000 Western Blot: 1:500- 1:3000. Immunohistochemistry: 1:300- 1:1500. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice