Biotin (Cy5)

Size

£1,023.00

SKU:: B1750-17E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Format:: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy5. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in FLISA and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Excitation Wavelength: 650nm Emission Wavelength: 667nm Fluorochrome/Protein Ratio: 5.3 moles Cy5 per mole of goat IgG F(ab’)2. Molecular weight of label: 975D. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Cy5 conjugates are sensitive to light.