Bin1 - 2BScientific

SKU:

B1200

Purity:

Purified by Protein G affinity chromatography.

Storage Conditions:

-20°C

Supplier:

United States Biological

Host:

Mouse

Immunogen:

Partial fusion protein corresponding to aa189-398 of human Bin1.

Clone:

0.T.07 (99D)

Format:

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Extra Details:

Bin1 is a ubiquitously expressed protein that interacts with the N-terminal region of the oncoprotein Myc. It inhibits cell transformation by Myc and has been implicted in Myc-mediated apoptosis. Bin1 is structurally related to amphiphysin, a breast cancer associated autoimmune antigen, and RVS167, a negative regulator of cell cycle in yeast. Bin1 is frequently absent in carcinoma cells and the gene maps to a hot spot for deletion in prostate cancer. Thus Bin1 has the features of a tumor suppressor and may play a role in apoptosis or cell cycle regulation. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitaton and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects Bin1 in a RIPA lysate of cells. Immunohistochemistry: Gives positive staining for Bin1 in C2C12, LNCap, HeLa, and Rat1 cells. Optimal dilutions to be determined by the researcher. *Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.