B1200-HRP
Bin1 (HRP)
Size
£1,190.00
- SKU:
- B1200-HRP
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Partial fusion protein corresponding to aa189-398 of human Bin1.
- Clone:
- 0.T.07 (99D)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Bin1 is a ubiquitously expressed protein that interacts with the N-terminal region of the oncoprotein Myc. It inhibits cell transformation by Myc and has been implicted in Myc-mediated apoptosis. Bin1 is structurally related to amphiphysin, a breast cancer associated autoimmune antigen, and RVS167, a negative regulator of cell cycle in yeast. Bin1 is frequently absent in carcinoma cells and the gene maps to a hot spot for deletion in prostate cancer. Thus Bin1 has the features of a tumor suppressor and may play a role in apoptosis or cell cycle regulation. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitaton and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: detects Bin1 in a RIPA lysate of cells. Immunohistochemistry: Gives positive staining for Bin1 in C2C12, LNCap, HeLa, and Rat1 cells. Optimal dilutions to be determined by the researcher. *Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice