B1095-03
BID (BH3 Interacting Domain Death Agonist)
Size
£655.00
- SKU:
- B1095-03
- Application:
- WB
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to 14 amino acids near the middle of human Bid (GenBank accession no. AAH36364)
- Format:
- Supplied as a liquid in PBS containing 0.02% sodium azide.
- Extra Details:
- Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3). Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals (4,5), exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein (6). The cleaved complex is myristoylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize (7,8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice