B1095-01W

BID, phosphorylated (Ser61) (Bcl-2 Interacting Domain, BH3 Interacting Domain Death Agonist)

Size

£831.00

SKU:: B1095-01W
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human Bid. Cellular Localization: Cytoplasm. Mitochondrion membrane.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.